murine tumor cell lines ct26  (ATCC)

Journal: Cancer Immunology Research

Article Title: High Levels of Endogenous Omega-3 Fatty Acids Promote Dendritic Cell Antigen Presentation and Improve Dendritic Cell–Based Cancer Vaccine Efficacy in Mice

doi: 10.1158/2326-6066.CIR-24-0927

Figure Lengend Snippet: FAT-1 transgenic mice show improved overall survival, lower body mass, and protection against tumor growth compared with WT mice. A, Schematic of the FAT-1 transgenic mouse model. B, Kaplan–Meier curve showing the survival probability of WT and FAT-1 naïve animals. C, Comparison of body weight of WT and FAT-1 animals as they age (****, P < 0.0005). D, Tumor growth kinetics of TC-1 lung cancer cell lines in FAT-1 and WT (*, P < 0.02). E, Kaplan–Meier survival curve for TC-1 lung cancer tumor–bearing WT and FAT-1 mice (**, P < 0.009). F, Tumor growth kinetics of B16F10 melanoma cancer cell lines in WT and FAT-1 animals (***, P < 0.0001). G, Kaplan–Meier survival curve for B16F10 melanoma tumor–bearing WT and FAT-1 mice (*, P < 0.002). H, Percentage of tumor-free mice after inoculation in WT vs. FAT-1 in the B16F10 melanoma tumor model (****, P < 0.005). I, Illustration showing the experimental design of BMDC culture and antigen presentation assay in vitro . J, The bar graph showing the frequency of major immune cell types in the spleen of WT vs. FAT-1 mice (orange, stained control; green, FAT-1; blue, WT). K, Levels of IFNγ in supernatant from WT or FAT-1 BMDCs cocultured with CD8 + T cells from PMEL-1 mice measured by ELISA (*, P < 0.008). L, Overlay of expression of major surface markers, including activation and costimulatory markers (CD11b, CD11c, MHC class II, CD40, CD80, CD86) between mature antigen-pulsed BMDCs derived from FAT-1 (green curve) and WT (blue curve) mice. Statistical analysis: Multiple comparisons were made using two-way ANOVA. The Šidák correction test was applied to correct for multiple comparisons. The Mann–Whitney test (unpaired/nonparametric comparing the difference in median) was used as applicable for all figures. EPA, eicosapentaenoic acid; PMA/ION, phorbol 12-myristate 13-acetate/ionomycin.

Article Snippet: B16F10 melanoma murine cancer cell line (ATCC, CRL-6475, 2019) was grown in complete RPMI 1640 (cRPMI), comprising RPMI 1640 supplemented with 10% FBS, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 2 mmol/L L-glutamine at 37°C in humidified air with 5% CO 2 .

Techniques: Transgenic Assay, Comparison, Immunopeptidomics, In Vitro, Staining, Control, Enzyme-linked Immunosorbent Assay, Expressing, Activation Assay, Derivative Assay, MANN-WHITNEY

Journal: Cancer Immunology Research

Article Title: High Levels of Endogenous Omega-3 Fatty Acids Promote Dendritic Cell Antigen Presentation and Improve Dendritic Cell–Based Cancer Vaccine Efficacy in Mice

doi: 10.1158/2326-6066.CIR-24-0927

Figure Lengend Snippet: FAT-1–derived BMDCs significantly reduce tumor burden in WT B16F10 melanoma tumor–bearing mice. A, Tumor inoculation and vaccination schedule. B, Kaplan–Meier survival curve for vehicle-, WT DC–, and FAT-1 DC–vaccinated B16F10 melanoma tumor–bearing animals. C, Tumor growth kinetic comparison of the vehicle- and WT DC–vaccinated tumor-bearing WT animals. D, Tumor growth kinetic comparison of the vehicle- and FAT-1 DC–vaccinated tumor-bearing WT animals. E–G, Tumor growth rate between day 10 (before vaccine dose 2) and day 17 (before vaccine dose 3) for vehicle-, WT DC–, or FAT-1 DC–vaccinated B16F10 melanoma tumor–bearing animals. H, Proportion of mice with smaller tumors (<600 mm 3 in TC-1 lung cancer and <500 mm 3 in B16F10 melanoma) in different DC vaccine groups in TC-1 lung cancer and B16F10 melanoma tumor–bearing mice. **, P < 0.005; ***, P < 0.0005; NS, nonsignificant. veh, vehicle.

Article Snippet: B16F10 melanoma murine cancer cell line (ATCC, CRL-6475, 2019) was grown in complete RPMI 1640 (cRPMI), comprising RPMI 1640 supplemented with 10% FBS, 100 IU/mL penicillin, 100 μg/mL streptomycin, and 2 mmol/L L-glutamine at 37°C in humidified air with 5% CO 2 .

Techniques: Derivative Assay, Comparison